Seurat 4.0.0 (2020-01-27) 2021-01-29

Added

Changes

  • Default neighbor finding algorithm changed from “rann” to “annoy”
  • Default ncells parameter in SCTransform() changed to 5000
  • Default fold change in FindMarkers() changed from ln to log2
  • Implementation improvements to AverageExpression()
  • AnchorSet class re-implemented as a virtual class from which IntegrationAnchorSet and TransferAnchorSet now inherit.
  • Point size in VlnPlot() now set automatically if not specified
  • Return the sample.tree properly when integrating with a single reference dataset
  • Replace as.character.quosure usage with as_label due to deprecation
  • Minor changes to the exact calculation of the anchor weight matrix
  • Default rasterization limit in DimPlot() and FeaturePlot() changed from 50,000 to 100,000
  • SCTransform() now returns a formalized Assay subclass SCTAssay()
  • When using normalization.method='SCT' in FindTransferAnchors(), normalize query using reference SCT model when possible.

Removed

Seurat 3.2.3 - 2020-12-14 2020-12-15

Added

  • Titles added to DimPlot when specifying group.by parameter
  • keep.scale parameter added to FeaturePlot to control scaling across multiple features and/or splits.

Changes

  • Same deprecated in favor of base::identity
  • Fix in DietSeurat to work with specialized Assay objects
  • Fix p-value return when using the ape implementation of Moran’s I
  • Fix bug in FindMarkers when using MAST with a latent variable
  • Updates to Key<-.DimReduc that allow handling of empty reduction column names
  • Allow setting ctrl in CellCycleScoring
  • Modify subset.Seurat to allow specialized Assay subsetting methods
  • Fix image selection in interactive spatial plots
  • Update Rcpp functions with export(rng=FALSE) to avoid potential future warnings
  • Fix RenameCells bug for integrated SCT assays
  • Fix highlight order with proper factor levels when using SetHighlight in plots
  • Small change in CellRanger version detection logic of h5 file to improve robustness to outside tools.
  • do.cpp deprecated and will default to true

Seurat 3.2.2 (2020-09-25) 2020-09-26

Changes

  • Set the seed in WhichCells regardless of whether or not idents is passed
  • Retain Graph and Neighbor objects when subsetting only on features
  • Fix data.frame input to CreateAssayObject() when data.frame has no rownames.
  • Default annoy search to sequential if not using multicore future plans.
  • Require sctransform >= 0.3.0

Seurat 3.2.1 (2020-09-04) 2020-09-07

Added

  • Added support for nearest neighbor input and return.model parameter in RunUMAP()
  • Enable named color vectors in DoHeatmap()
  • Add label.color and label.box parameters to DimPlot
  • Added shuffle and seed parameters to DimPlot() to help with overplotting
  • Added new stacked violin plot functionality

Changes

  • Allow setting slot parameter in RunUMAP
  • Added support for FIt-SNE v1.2+
  • Fix for Spatial*Plot when running with interactive=TRUE
  • Set max for number of items returned by Top and remove duplicate items when balanced=TRUE
  • Fix logging bug when functions were run via do.call()
  • Fix handling of weight.by.var parameter when approx=FALSE in RunPCA()
  • Fix issue where feature names with dashes crashed CellSelector
  • Fix issue where errors in subsetting were being swallowed
  • Fix issue where labeling uncropped spatial plots was broken

Deprecated

  • CreateActivityMatrix deprecated in favor of Signac::GeneActivity
  • ReadAlevin and ReadAlevinCsv deprecated in favor of SeuratWrappers::ReadAlevin
  • ExportToCellbrowser and StopCellbrowser deprecated in favor of SeuratWrappers::ExportToCellbrowser and SeuratWrappers::StopCellbrowser
  • ReadH5AD and WriteH5AD deprecated in favor of h5Seurat/H5AD functionality found in SeuratDisk
  • as.loom and as.Seurat.loom deprecated in favor of functionality found in SeuratDisk

Seurat 3.2.0 (2020-07-15) 2020-07-16

Added

  • Added ability to create a Seurat object from an existing Assay object, or any object inheriting from the Assay class
  • Added ability to cluster idents and group features in DotPlot
  • Added ability to use RColorBrewer plaettes for split DotPlots
  • Added visualization and analysis functionality for spatially resolved datasets (Visium, Slide-seq).

Changes

  • Removed add.iter parameter from RunTSNE function
  • Fixed integer overflow error in the WilcoxDETest function
  • Minor visual fixes in DoHeatmap group bar + labels
  • Efficiency improvements in anchor scoring (ScoreAnchors)
  • Fix bug in FindClusters() when the last node has no edges
  • Default to weighted = TRUE when constructing igraph objects in RunLeiden. Remove corresponding weights parameter from FindClusters().
  • Fix handling of keys in FeatureScatter()
  • Change CellSelector to use Shiny gadgets instead of SDMTools
  • Mark PointLocator as defunct
  • Remove SDMTools
  • Fixed data slot return in AverageExpression when subsetting features and returning a Seurat object

Seurat 3.1.5 (2020-04-14) 2020-04-16

Added

  • New scale parameter in DotPlot
  • New keep.sparse parameter inCreateGeneActivityMatrix` for a more memory efficient option
  • Added ability to store model learned by UMAP and project new data
  • New strip.suffix option in Read10X. This changes the default behavior of Read10X. A trailing -1 present in all cell names will not be removed by default.
  • Added group.by parameter to FeatureScatter

Changes

  • Replace wilcox.test with limma implementation for a faster FindMarkers default method
  • Better point separation for VlnPlots when using the split.by option
  • Efficiency improvements for anchor pairing
  • Deprecate redundant sort.cell parameter in FeaturePlot
  • Fixes to ensure correct class of Matrix passed to c++ functions
  • Fixes for underscores in ident labels for DotPlot
  • Ensure preservation of matrix dimnames in SampleUMI
  • Fix non-standard evaluation problems in subset and WhichCells
  • Default split violin option is now a multi group option
  • Preserve alpha in FeaturePlot when using blend
  • Update assay.used slot for DimReducs when Assay is renamed

Seurat 3.1.4 (2020-02-20) 2020-02-26

Changes

  • Fixes to DoHeatmap to remain compatible with ggplot2 v3.3
  • Adoption of patchwork framework to replace CombinePlots

Seurat 3.1.3 (2020-02-07) 2020-02-11

Added

  • New system agnostic Which function to address problems with FItSNE on Windows

Changes

  • Export CellsByIdentities and RowMergeSparseMatrices functions
  • nCount and nFeature metadata variables retained after subset and updated properly with UpdateSeuratObject
  • Fix uwot support for running directly on feature matrices
  • Fixes for keys with underscores
  • Fix issue with leiden option for FindClusters
  • Fix for data transfer when using sctransform
  • SDMTools moved to Suggests as package is orphaned

Seurat 3.1.2 (2019-12-11) 2019-12-12

Added

  • New silent slot updater
  • New random seed options to RunCCA, RunTSNE, WhichCells, HTODemux, AddModuleScore, VlnPlot, and RidgePlot
  • Enhancements for dealing with Assay-derived objects

Changed

  • Only run CalcN (generates nFeatures and nCounts) when counts changes
  • Fix issue regarding colons in feature names
  • Change object class testing to use inherits or is.* for R 4.0 compatability

Seurat 3.1.1 (2019-09-20) 2019-10-03

Added

  • New RegroupIdents function to reassign idents based on metadata column majority
  • UpdateSymbolList function to pull new gene names from HGNC
  • Added support for H5AD layers as additional assays in a Seurat object

Changed

  • Fix rownames issue when running UMAP on dist object
  • Add support for new H5AD obsm and varm stucture
  • Fix issue when trying to read non-existent feature-level metadata from an H5AD file
  • Fix in integration workflow when using SCTransform
  • Improved error checking for AddModuleScore
  • cbind fix in reference-based integration (MapQuery)
  • Fix for convenience plots error hanging
  • Ensure Seurat objects aren’t stored in the command logs

Seurat 3.1.0 (2019-08-20) 2019-08-20

Added

  • New PrepSCTIntegration function to facilitate integration after SCTransform
  • Reference-based integration with the reference parameter in FindIntegrationAnchors
  • Reciprocal PCA as a reduction option in FindIntegrationAnchors
  • New CollapseEmbeddingOutliers function
  • Enable FindTransferAnchors after SCTransform
  • Added back ColorDimSplit functionality
  • Include a code of conduct
  • Added uwot support as new default UMAP method
  • Added CheckDots to catch unused parameters and suggest updated names
  • Reductions and Assays assays functions to list stored DimReducs and Assays

Changed

  • Fix regex in LogSeuratCommand
  • Check for NAs in feature names in Read10X
  • Prevent dimnames for counts/data/scale.data matrices from being arrays
  • Updates ReadH5AD to distinguish FVF methods
  • Fixes to UpdateSeuratObject for v2 objects
  • Sink all output from stdout to stderr
  • Fix to scale.data cell ordering after subsetting
  • Enable Assay specification in BuildClusterTree
  • Fix FeaturePlot when using both blend and split.by
  • Fix to WhichCells when passing cells and invert
  • Fix to HoverLocator labels and title
  • Ensure features names don’t contain pipes (|)
  • Deprecation of RunLSI and RunALRA
  • Fix legend bug when sorting in ExIPlot

Seurat 3.0.2 (2019-06-07) 2019-06-14

Added

  • Flag to skip singleton grouping in FindClusters
  • New custom colors for blended FeaturePlots
  • New GetResidual function
  • New Seurat/Monocle converters

Changed

  • Fix issue where certain assays weren’t being shown in the Seurat object
  • Fix issue where we weren’t updating DimReduc object column names
  • Fix line spacers in DoHeatmap
  • Fix uninformative labels in FeaturePlot
  • Fix unset identities when converting from SCE to Seurat
  • Fix single colors being interpreted as palettes in SingleDimPlot
  • Ensure factor levels are always numerically increasing after FindClusters
  • Better cell highlighting colors for DimPlot
  • Fix to levels<-.Seurat
  • Add ability to use counts/scaled data in BuildClusterTree
  • Minor fix to split ScaleData

Seurat 3.0.1 (2019-05-16) 2019-05-16

Added

  • Add global option (Seurat.memsafe) to skip gc() calls
  • Restore draw.lines to DoHeatmap, maintain size of color bar with different number of features (#1429)
  • Enable split.by parameter for ScaleData
  • Add slot parameter to FeaturePlot (#1483)
  • Add assay parameter to DotPlot (#1404)

Changed

  • Fix to color options for VlnPlot with split.by option (#1425)
  • Improvements to conversion functions (loom, SCE)
  • Fix for cluster tree reordering (#1434)
  • Fix PercentageFeatureSet for single feature case
  • Fix to fold change calculation and filtering for other slots in FindMarkers (#1454)
  • Keep title vectorized in AugmentPlot (#1515)
  • Export LogSeuratCommand function
  • Fix for FindConservedMarkers when one ident is missing from a group (#1517)

Seurat 3.0.0 (2019-04-16) 2019-04-15

Added

  • New method for identifying anchors across single-cell datasets
  • Parallelization support via future
  • Additional method for demultiplexing with MULTIseqDemux
  • Support normalization via sctransform
  • New option for clustering with the Leiden algorithm
  • Support for reading 10X v3 files
  • New function to export Seurat objects for the UCSC cell browser
  • Support for data import from Alevin outputs
  • Imputation of dropped out values via ALRA

Changed

  • Significant code restructuring
  • Most occurances of “gene(s)” in function names/arguments renamed to “feature(s)”
  • Changes to the Seurat object class to facilitate multimodal data
  • New BlendPlot implementation

Seurat 2.3.4 (2018-07-13) 2018-07-17

Added

  • GetIdent function added to pull identity info

Changed

  • DiffusionMap dependency replaced with destiny to avoid archival
  • Java dependency removed and functionality rewritten in Rcpp
  • Speed and efficiency improvements for Rcpp code
  • More robust duplicate handling in CellCycleScoring

Seurat 2.3.3 (2018-07-02) 2018-07-02

Added

  • New HTOHeatmap function
  • Support for custom PNG arguments for vector-friendly plotting
  • Fix for ‘NA’-labeled cells disappearing with custom color scale

Changed

  • Replaced FNN with RANN
  • Removed unused compiler flags
  • Moved several lightly-used packages from ‘imports’ to ‘suggests’

Seurat 2.3.2 (2018-06-11) 2018-06-12

Added

  • RenameCells added for easy renaming of all cells
  • Read10X_h5 added to read in 10X formatted h5 files
  • SetAssayData ensures cell order is the same between assay objects and the Seurat object
  • Compatability updates for ggplot2 v2.3.0

Seurat 2.3.1 (2018-05-03) 2018-05-05

Added

  • Support for UMAP dimensional reduction technique
  • New conversion functions for SingleCellExperiment and anndata

Changed

  • FetchData preserves cell order
  • Require Matrix 1.2-14 or higher
  • AddModuleScore no longer densifies sparse-matrices
  • Various visualization fixes and improvements
  • Default value for latent.vars in FindMarkers/FindAllMarkers changed to NULL.

Seurat 2.3.0 (2018-03-22) 2018-03-23

Added

  • Support for HTO demultiplexing
  • Utility functions: TransferIdent, CombineIdent, SplitObject, vector.friendly
  • C++ implementation for parts of BuildSNN
  • Preliminary parallelization support (regression and JackStraw)
  • Support for FItSNE

Changed

  • MetaDE replaced with metap for combining p-values (MetaDE was removed from CRAN)
  • NMF heatmaps replaced (NMF to be archived by CRAN)

Seurat 2.2.1 (2018-02-14) 2018-02-14

Changed

  • MetaDE replaced with metap for combining p-values (MetaDE was removed from CRAN)
  • NMF heatmaps replaced (NMF to be archived by CRAN)

Seurat 2.2.0 (2018-01-10) 2018-01-10

Added

  • Multiple alignment functionality with RunMultiCCA and AlignSubspace extended to multiple datasets
  • CalcAlignmentScore added to evaluate alignment quality
  • MetageneBicorPlot added to guide CC selection
  • Change cluster order in DoHeatmap with group.order parameter
  • Ability to change plotting order and add a title to DimPlot
  • do.clean and subset.raw options for SubsetData

Changed

  • JoyPlot has been replaced with RidgePlot
  • FindClusters is now more robust in making temp files
  • MetaDE support for combining p-values in DE testing

Seurat 2.1.0 (2017-10-12) 2017-10-12

Added

  • Support for using MAST and DESeq2 packages for differential expression testing in FindMarkers
  • Support for multi-modal single-cell data via @assay slot

Changed

  • Default DE test changed to Wilcoxon rank sum test

Seurat 2.0.1 (2017-08-18) 2017-08-22

Added

  • Now available on CRAN
  • Updated documentation complete with examples
  • Example datasets: pbmc_small and cc.genes
  • C++ implementation for parts of FindVariableGenes
  • Minor bug fixes

Seurat 2.0.0 (2017-07-26) Unreleased

Added

  • New method for aligning scRNA-seq datasets
  • Significant code restructuring
  • New methods for scoring gene expression and cell-cycle phases
  • New visualization features (do.hover, do.identify)