Find a set of anchors between a reference and query object. These anchors can later be used to transfer data from the reference to query object using the TransferData object.

  normalization.method = "LogNormalize",
  recompute.residuals = TRUE,
  reference.assay = NULL,
  reference.neighbors = NULL,
  query.assay = NULL,
  reduction = "pcaproject",
  reference.reduction = NULL,
  project.query = FALSE,
  features = NULL,
  npcs = 30,
  l2.norm = TRUE,
  dims = 1:30,
  k.anchor = 5,
  k.filter = 200,
  k.score = 30,
  max.features = 200,
  nn.method = "annoy",
  n.trees = 50,
  eps = 0,
  approx.pca = TRUE,
  mapping.score.k = NULL,
  verbose = TRUE



Seurat object to use as the reference


Seurat object to use as the query


Name of normalization method used: LogNormalize or SCT.


If using SCT as a normalization method, compute query Pearson residuals using the reference SCT model parameters.


Name of the Assay to use from reference


Name of the Neighbor to use from the reference. Optionally enables reuse of precomputed neighbors.


Name of the Assay to use from query


Dimensional reduction to perform when finding anchors. Options are:

  • pcaproject: Project the PCA from the reference onto the query. We recommend using PCA when reference and query datasets are from scRNA-seq

  • cca: Run a CCA on the reference and query


Name of dimensional reduction to use from the reference if running the pcaproject workflow. Optionally enables reuse of precomputed reference dimensional reduction.


Project the PCA from the query dataset onto the reference. Use only in rare cases where the query dataset has a much larger cell number, but the reference dataset has a unique assay for transfer. In this case, the default features will be set to the variable features of the query object that are alos present in the reference.


Features to use for dimensional reduction. If not specified, set as variable features of the reference object which are also present in the query.


Number of PCs to compute on reference if reference.reduction is not provided.


Perform L2 normalization on the cell embeddings after dimensional reduction


Which dimensions to use from the reduction to specify the neighbor search space


How many neighbors (k) to use when finding anchors


How many neighbors (k) to use when filtering anchors. Set to NA to turn off filtering.


How many neighbors (k) to use when scoring anchors


The maximum number of features to use when specifying the neighborhood search space in the anchor filtering


Method for nearest neighbor finding. Options include: rann, annoy


More trees gives higher precision when using annoy approximate nearest neighbor search


Error bound on the neighbor finding algorithm (from RANN)


Use truncated singular value decomposition to approximate PCA


Compute and store nearest k query neighbors in the AnchorSet object that is returned. You can optionally set this if you plan on computing the mapping score and want to enable reuse of some downstream neighbor calculations to make the mapping score function more efficient.


Print progress bars and output


Returns an AnchorSet object that can be used as input to TransferData


The main steps of this procedure are outlined below. For a more detailed description of the methodology, please see Stuart, Butler, et al Cell 2019. doi: 10.1016/j.cell.2019.05.031 ; doi: 10.1101/460147

  • Perform dimensional reduction. Exactly what is done here depends on the values set for the reduction and project.query parameters. If reduction = "pcaproject", a PCA is performed on either the reference (if project.query = FALSE) or the query (if project.query = TRUE), using the features specified. The data from the other dataset is then projected onto this learned PCA structure. If reduction = "cca", then CCA is performed on the reference and query for this dimensional reduction step. If l2.norm is set to TRUE, perform L2 normalization of the embedding vectors.

  • Identify anchors between the reference and query - pairs of cells from each dataset that are contained within each other's neighborhoods (also known as mutual nearest neighbors).

  • Filter low confidence anchors to ensure anchors in the low dimension space are in broad agreement with the high dimensional measurements. This is done by looking at the neighbors of each query cell in the reference dataset using max.features to define this space. If the reference cell isn't found within the first k.filter neighbors, remove the anchor.

  • Assign each remaining anchor a score. For each anchor cell, determine the nearest k.score anchors within its own dataset and within its pair's dataset. Based on these neighborhoods, construct an overall neighbor graph and then compute the shared neighbor overlap between anchor and query cells (analogous to an SNN graph). We use the 0.01 and 0.90 quantiles on these scores to dampen outlier effects and rescale to range between 0-1.


Stuart T, Butler A, et al. Comprehensive Integration of Single-Cell Data. Cell. 2019;177:1888-1902 doi: 10.1016/j.cell.2019.05.031 ;


if (FALSE) { # to install the SeuratData package see library(SeuratData) data("pbmc3k") # for demonstration, split the object into reference and query pbmc.reference <- pbmc3k[, 1:1350] pbmc.query <- pbmc3k[, 1351:2700] # perform standard preprocessing on each object pbmc.reference <- NormalizeData(pbmc.reference) pbmc.reference <- FindVariableFeatures(pbmc.reference) pbmc.reference <- ScaleData(pbmc.reference) pbmc.query <- NormalizeData(pbmc.query) pbmc.query <- FindVariableFeatures(pbmc.query) pbmc.query <- ScaleData(pbmc.query) # find anchors anchors <- FindTransferAnchors(reference = pbmc.reference, query = pbmc.query) # transfer labels predictions <- TransferData( anchorset = anchors, refdata = pbmc.reference$seurat_annotations ) pbmc.query <- AddMetaData(object = pbmc.query, metadata = predictions) }