This function will construct a weighted nearest neighbor (WNN) graph. For each cell, we identify the nearest neighbors based on a weighted combination of two modalities. Takes as input two dimensional reductions, one computed for each modality.Other parameters are listed for debugging, but can be left as default values.

FindMultiModalNeighbors( object, reduction.list, dims.list, k.nn = 20, l2.norm = TRUE, knn.graph.name = "wknn", snn.graph.name = "wsnn", weighted.nn.name = "weighted.nn", modality.weight.name = NULL, knn.range = 200, prune.SNN = 1/15, sd.scale = 1, cross.contant.list = NULL, smooth = FALSE, return.intermediate = FALSE, modality.weight = NULL, verbose = TRUE )

object | A Seurat object |
---|---|

reduction.list | A list of two dimensional reductions, one for each of the modalities to be integrated |

dims.list | A list containing the dimensions for each reduction to use |

k.nn | the number of multimodal neighbors to compute. 20 by default |

l2.norm | Perform L2 normalization on the cell embeddings after dimensional reduction. TRUE by default. |

knn.graph.name | Multimodal knn graph name |

snn.graph.name | Multimodal snn graph name |

weighted.nn.name | Multimodal neighbor object name |

modality.weight.name | Variable name to store modality weight in object meta data |

knn.range | The number of approximate neighbors to compute |

prune.SNN | Cutoff not to discard edge in SNN graph |

sd.scale | The scaling factor for kernel width. 1 by default |

cross.contant.list | Constant used to avoid divide-by-zero errors. 1e-4 by default |

smooth | Smoothing modality score across each individual modality neighbors. FALSE by default |

return.intermediate | Store intermediate results in misc |

modality.weight | A |

verbose | Print progress bars and output |

Seurat object containing a nearest-neighbor object, KNN graph, and SNN graph - each based on a weighted combination of modalities.