Single cell violin plot

Draws a violin plot of single cell data (gene expression, metrics, PC scores, etc.)

Usage

VlnPlot(
  object,
  features,
  cols = NULL,
  pt.size = NULL,
  alpha = 1,
  idents = NULL,
  sort = FALSE,
  assay = NULL,
  group.by = NULL,
  split.by = NULL,
  adjust = 1,
  y.max = NULL,
  same.y.lims = FALSE,
  log = FALSE,
  ncol = NULL,
  slot = deprecated(),
  layer = NULL,
  split.plot = FALSE,
  stack = FALSE,
  combine = TRUE,
  fill.by = "feature",
  flip = FALSE,
  add.noise = TRUE,
  raster = NULL,
  raster.dpi = 300
)

Arguments

object

Seurat object

features

Features to plot (gene expression, metrics, PC scores, anything that can be retreived by FetchData)

cols

Colors to use for plotting

pt.size

Point size for points

alpha

Alpha value for points

idents

Which classes to include in the plot (default is all)

sort

Sort identity classes (on the x-axis) by the average expression of the attribute being potted, can also pass 'increasing' or 'decreasing' to change sort direction

assay

Name of assay to use, defaults to the active assay

group.by

Group (color) cells in different ways (for example, orig.ident)

split.by

A factor in object metadata to split the plot by, pass 'ident' to split by cell identity

adjust

Adjust parameter for geom_violin

y.max

Maximum y axis value

same.y.lims

Set all the y-axis limits to the same values

log

plot the feature axis on log scale

ncol

Number of columns if multiple plots are displayed

slot

Slot to pull expression data from (e.g. "counts" or "data")

layer

Layer to pull expression data from (e.g. "counts" or "data")

split.plot

plot each group of the split violin plots by multiple or single violin shapes.

stack

Horizontally stack plots for each feature

combine

Combine plots into a single patchworked ggplot object. If FALSE, return a list of ggplot

fill.by

Color violins/ridges based on either 'feature' or 'ident'

flip

flip plot orientation (identities on x-axis)

add.noise

determine if adding a small noise for plotting

raster

Convert points to raster format. Requires 'ggrastr' to be installed.

raster.dpi

the dpi for raster layer, default is 300. See rasterize for more info.

Value

A patchworked ggplot object if combine = TRUE; otherwise, a list of ggplot objects

See also

FetchData

Examples

data("pbmc_small")
VlnPlot(object = pbmc_small, features = 'PC_1')
VlnPlot(object = pbmc_small, features = 'LYZ', split.by = 'groups')