Load in the data

This vignette demonstrates new features that allow users to analyze and explore multi-modal data with Seurat. While this represents an initial release, we are excited to release significant new functionality for multi-modal datasets in the future.

Here, we analyze a dataset of 8,617 cord blood mononuclear cells (CBMCs), produced with CITE-seq, where we simultaneously measure the single cell transcriptomes alongside the expression of 11 surface proteins, whose levels are quantified with DNA-barcoded antibodies. First, we load in two count matrices : one for the RNA measurements, and one for the antibody-derived tags (ADT). You can download the ADT file here and the RNA file here

# Load in the RNA UMI matrix

# Note that this dataset also contains ~5% of mouse cells, which we can use as negative controls
# for the protein measurements. For this reason, the gene expression matrix has HUMAN_ or MOUSE_
# appended to the beginning of each gene.
cbmc.rna <- as.sparse(read.csv(file = "../data/GSE100866_CBMC_8K_13AB_10X-RNA_umi.csv.gz", sep = ",", 
    header = TRUE, row.names = 1))

# To make life a bit easier going forward, we're going to discard all but the top 100 most
# highly expressed mouse genes, and remove the 'HUMAN_' from the CITE-seq prefix
cbmc.rna <- CollapseSpeciesExpressionMatrix(cbmc.rna)

# Load in the ADT UMI matrix
cbmc.adt <- as.sparse(read.csv(file = "../data/GSE100866_CBMC_8K_13AB_10X-ADT_umi.csv.gz", sep = ",", 
    header = TRUE, row.names = 1))

# When adding multimodal data to Seurat, it's okay to have duplicate feature names. Each set of
# modal data (eg. RNA, ADT, etc.) is stored in its own Assay object.  One of these Assay objects
# is called the 'default assay', meaning it's used for all analyses and visualization.  To pull
# data from an assay that isn't the default, you can specify a key that's linked to an assay for
# feature pulling.  To see all keys for all objects, use the Key function.  Lastly, we observed
# poor enrichments for CCR5, CCR7, and CD10 - and therefore remove them from the matrix
# (optional)
cbmc.adt <- cbmc.adt[setdiff(rownames(x = cbmc.adt), c("CCR5", "CCR7", "CD10")), ]

Setup a Seurat object, and cluster cells based on RNA expression

The steps below represent a quick clustering of the PBMCs based on the scRNA-seq data. For more detail on individual steps or more advanced options, see our PBMC clustering guided tutorial here

cbmc <- CreateSeuratObject(counts = cbmc.rna)

# standard log-normalization
cbmc <- NormalizeData(cbmc)

# choose ~1k variable features
cbmc <- FindVariableFeatures(cbmc)

# standard scaling (no regression)
cbmc <- ScaleData(cbmc)

# Run PCA, select 13 PCs for tSNE visualization and graph-based clustering
cbmc <- RunPCA(cbmc, verbose = FALSE)
ElbowPlot(cbmc, ndims = 50)

cbmc <- FindNeighbors(cbmc, dims = 1:25)
cbmc <- FindClusters(cbmc, resolution = 0.8)
cbmc <- RunTSNE(cbmc, dims = 1:25, method = "FIt-SNE")

# Find the markers that define each cluster, and use these to annotate the clusters, we use
# max.cells.per.ident to speed up the process
cbmc.rna.markers <- FindAllMarkers(cbmc, max.cells.per.ident = 100, min.diff.pct = 0.3, only.pos = TRUE)
# Note, for simplicity we are merging two CD14+ Monocyte clusters (that differ in expression of
# HLA-DR genes) and NK clusters (that differ in cell cycle stage)
new.cluster.ids <- c("Memory CD4 T", "CD14+ Mono", "Naive CD4 T", "NK", "CD14+ Mono", "Mouse", "B", 
    "CD8 T", "CD16+ Mono", "T/Mono doublets", "NK", "CD34+", "Multiplets", "Mouse", "Eryth", "Mk", 
    "Mouse", "DC", "pDCs")
names(new.cluster.ids) <- levels(cbmc)
cbmc <- RenameIdents(cbmc, new.cluster.ids)
DimPlot(cbmc, label = TRUE) + NoLegend()