Single Cell Genomics Day: A Practical Workshop
Recent developments in molecular biology, microfluidics, and computational biology have transformed the field of single cell genomics. However, the breathtaking pace of technology development has given rise to a multitude of molecular protocols, commercial systems, and computational challenges.
The Satija Lab is excited to host the Second Annual Single Cell Genomics Day on Friday, January 19, 2018. This workshop will begin with an overview of exciting developments in the field over the past year, followed by in-depth presentations with an emphasis on practical details and considerations. Our goal is not to fully describe the details of each method, but instead to highlight the intuition and key use cases that can help motivate new users to get started.
Single Cell Genomics Day will be held at the NYU Center for Genomics and Systems Biology, with check in beginning at 9:00 AM. Please register in advance to attend in person. The workshop is free for all participants, thanks to generous support from the NYU Biology department and Center for Genomics and Systems Biology.
We thank the Chan Zuckerberg Initiative for supporting a livestream of the presentation. The livestream has ended and is not available for viewing.
Agenda
| 9:30 — 10:00 | Rahul Satija
NYGC/NYU Download Slides, Summary and References Single Cell Genomics: Recent Advances and Future Directions View the talk here |
| 10:00 — 10:30 | Marlon Stoeckius
NYGC Technology Innovation Lab Download Slides Simultaneous Measurement of mRNA and Protein in Single Cells Topics: CITE-Seq, Abseq, REAP-Seq |
| 10:30 — 11:00 | Shiwei Zheng
NYGC/NYU Download Slides Multiplexing Strategies for Single Cell Sequencing Topics: Genetic Demultiplexing, Cell 'Hashing' |
| 11:00 — 11:15 | Coffee Break |
| 11:15 — 12:00 | Evan Macosko
Broad Institute Keynote: Molecular Exploration of the Brain at Single Cell Resolution |
| 12:00 — 12:30 | Kasandra Burgos
10X Genomics Expanding the Range of Applications for Single Cell RNA-Seq Topics: Sample Fixation, 5' Sequencing, Nuclear Profiling |
| 12:30 — 13:30 | Lunch |
| 13:30 — 14:00 | Jan Slabbaert
NYGC/NYU Download Slides Applying Single Cell Sequencing for Lineage Reconstruction Topics: GESTALT, CRISPR scars |
| 14:00 — 14:45 | Peter Kharchenko
Harvard Medical School Keynote: Transcriptional Dynamics in Single Cells |
| 14:45 — 15:00 | Coffee Break |
| 15:00 — 15:25 | Andrew Butler
NYGC/NYU Download Slides Batch Correction and Data Integration for Single Cell Transcriptomics |
| 15:25 — 15:50 | Efthymia Papalexi
NYGC/NYU Download Slides Combining CRISPR Screens with Single Cell RNA-Seq Topics: Perturb-Seq, CROP-Seq, CRISP-seq |
Map
Additional resources
Below, we list a few useful resources for those who would like to a brief introduction to the field prior to the workshop.